Catalog Number - 40031-05P

40031-05P Cogent UDA™ (weak cation exchange, WCX) HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), delivering a highly unique and flexible mixed‑mode separation mechanism not achievable with conventional C18 or traditional ion exchange columns.

The UDA™ ligand (Undecanoic Acid) consists of an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling multiple retention mechanisms:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This combination provides powerful selectivity for basic, polar, and ionizable compounds, particularly in complex matrices where co‑elution is common.

The TYPE‑C silica base offers distinct performance advantages:

• Direct silicon–carbon bonding for exceptional durability

• Ability to operate at very low pH (<2) without damage

• Faster equilibration compared to conventional silica phases

• Reduced sensitivity to mobile phase moisture

50 mm Length – Fast, High‑Throughput Analytical Separations

The 50 mm length is optimized for speed and efficiency in analytical HPLC workflows.

• Short run times and rapid throughput

• Reduced solvent consumption

• Ideal for screening and routine analysis

✅ Recommended for high‑throughput screening, QC labs, and LC‑MS workflows

Flow Rate & System Requirements (4.6 mm ID, 4 µm)

✅ Compatible with standard HPLC systems

• Typical flow rate range: ~0.8 – 1.5 mL/min

• Moderate backpressure due to 4 µm particle size

• Compatible with standard UV and MS detectors

• Suitable for routine analytical workflows

✅ Best Practice: Use proper sample filtration and guard column protection for optimal performance

Unique Selectivity – Why Choose UDA™ (WCX)

True Multi‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Ideal for Complex Matrices

• Resolves co‑eluting compounds

• Effective for basic and positively charged analytes

pH‑Controlled Retention

• WCX functionality active at pH ≥ 6

• Selectivity can be tuned using pH and ionic strength

✅ Ideal when traditional C18 or ion exchange columns do not provide adequate separation

TYPE‑C Silica Advantages

Extreme Robustness

• Stable under harsh conditions, including low pH

• Resistant to hydrolysis

Faster Equilibration

• Reduces total analysis time

No Strict Moisture Control (HILIC)

• Simplifies method development

Long Column Lifetime

• Durable chemistry for routine use

Cogent UDA™ Column Advantages

Mixed‑Mode Flexibility

• Combines RP, HILIC, and WCX in one column

Balanced Performance (4 µm Particles)

• Compatible with standard HPLC systems

• Good efficiency with moderate backpressure

High‑Throughput Ready

• Optimized for routine lab workflows

Typical Applications

• High‑throughput analytical screening

• LC‑MS method development

• Basic and charged compound separations

• Polar and ionizable analytes

• Pharmaceutical and bioanalytical workflows